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mouse cxcl14/brak elisa kit (colorimetric) (Novus Biologicals)

Name: mouse cxcl14/brak elisa kit (colorimetric)
Brand: Novus Biologicals
Rating: 90 (1 reviews)

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Novus Biologicals mouse cxcl14/brak elisa kit (colorimetric)
Mouse Cxcl14/Brak Elisa Kit (Colorimetric), supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse cxcl14/brak elisa kit (colorimetric)/product/Novus Biologicals
Average 90 stars, based on 1 article reviews

mouse cxcl14/brak elisa kit (colorimetric) - by Bioz Stars, 2026-02

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(A) H+E (100x, scale bar= 200 μm) and immunohistochemistry for CD68+ macrophages, p21, and CXCL14 (400x, scale bar=50 μm). For the p21 IHC the solid grey line represents the border of the area of necrosis, CV=central vein, showing that the p21+ hepatocytes border (red arrows) the necrotic area. (B) Heatmap of SASP-R-MMU-2559582 genes from bulk RNAseq of liver explant homogenate (data processing described in Methods). (C) Western blot and corresponding densitometry of CXCL14 and p21. (D-F) qPCR for Klf6, p21, and CXCL14 (n=2 for control, and n=1 for ALF). (E) UMAP representation of snRNAseq human APAP-ALF (n=11) or healthy (n=9) livers, with a bar graph showing the relative proportion of either healthy or APAP-ALF cells per cluster. (F) UMAP representation of the p21-secretome score (described in methods) showing high expression in cluster 7.

Journal: Toxicology

Article Title: The p21 + Perinecrotic Hepatocytes Produce the Chemokine CXCL14 After a Severe Acetaminophen Overdose Promoting Hepatocyte Injury and Delaying Regeneration

doi: 10.1016/j.tox.2024.153804

Figure Lengend Snippet: (A) H+E (100x, scale bar= 200 μm) and immunohistochemistry for CD68+ macrophages, p21, and CXCL14 (400x, scale bar=50 μm). For the p21 IHC the solid grey line represents the border of the area of necrosis, CV=central vein, showing that the p21+ hepatocytes border (red arrows) the necrotic area. (B) Heatmap of SASP-R-MMU-2559582 genes from bulk RNAseq of liver explant homogenate (data processing described in Methods). (C) Western blot and corresponding densitometry of CXCL14 and p21. (D-F) qPCR for Klf6, p21, and CXCL14 (n=2 for control, and n=1 for ALF). (E) UMAP representation of snRNAseq human APAP-ALF (n=11) or healthy (n=9) livers, with a bar graph showing the relative proportion of either healthy or APAP-ALF cells per cluster. (F) UMAP representation of the p21-secretome score (described in methods) showing high expression in cluster 7.

Article Snippet: ELISA Assay The mouse CXCL14 ELISA kit was purchased from Novus Biologicals (#70015).

Techniques: Immunohistochemistry, Western Blot, Control, Expressing

(A-B) Western blot and corresponding densitometry of Klf6 and p21. (C) qPCR for Klf6 and p21 in whole liver homogenate (control normalized to 1). (D) IHC for p21 (400x, scale bar= 50 μm). (E) qPCR for CXCL14 (control normalized to 1). (F) ELISA measuring CXCL14 in plasma. (G-H) IHC for PCNA (400x) and corresponding PCNA+ hepatocyte counts per HPF (200x, scale bar = 100 μm). Data are presented as mean ± SEM. Statistical significance was assessed by T-test for A300 vs A600 for each individual timepoint (B), and two-way ANOVA with Sidak’s post hoc test (C, E, F, H). N=4-5 per group, *p<0.05, **p<0.01.

Journal: Toxicology

Article Title: The p21 + Perinecrotic Hepatocytes Produce the Chemokine CXCL14 After a Severe Acetaminophen Overdose Promoting Hepatocyte Injury and Delaying Regeneration

doi: 10.1016/j.tox.2024.153804

Figure Lengend Snippet: (A-B) Western blot and corresponding densitometry of Klf6 and p21. (C) qPCR for Klf6 and p21 in whole liver homogenate (control normalized to 1). (D) IHC for p21 (400x, scale bar= 50 μm). (E) qPCR for CXCL14 (control normalized to 1). (F) ELISA measuring CXCL14 in plasma. (G-H) IHC for PCNA (400x) and corresponding PCNA+ hepatocyte counts per HPF (200x, scale bar = 100 μm). Data are presented as mean ± SEM. Statistical significance was assessed by T-test for A300 vs A600 for each individual timepoint (B), and two-way ANOVA with Sidak’s post hoc test (C, E, F, H). N=4-5 per group, *p<0.05, **p<0.01.

Article Snippet: ELISA Assay The mouse CXCL14 ELISA kit was purchased from Novus Biologicals (#70015).

Techniques: Western Blot, Control, Enzyme-linked Immunosorbent Assay, Clinical Proteomics

(A) Separate western blots for p21 at the 300 vs 600 dose shows transient p21 induction at 300 mg/kg APAP and (B) densitometry relative to control of Western blots shown in (A). (C) Western blot for p21 directly comparing 300 mg/kg and 600 mg/kg. (D) ELISA measuring CXCL14 plasma levels. (E) Immunohistochemistry for PCNA at 24 to 72h (200x) and corresponding PCNA+ hepatocyte counts per HPF (200x) (F). (G) Western blot of Klf6 and Cyclin D1 normalized to Gapdh. Data are presented as mean ± SEM. Statistical significance was assessed by two-way ANOVA with Sidak’s post hoc test. N=4-5 per group, *p<0.05, **p<0.01, ***p<0.001.

Journal: Toxicology

Article Title: The p21 + Perinecrotic Hepatocytes Produce the Chemokine CXCL14 After a Severe Acetaminophen Overdose Promoting Hepatocyte Injury and Delaying Regeneration

doi: 10.1016/j.tox.2024.153804

Figure Lengend Snippet: (A) Separate western blots for p21 at the 300 vs 600 dose shows transient p21 induction at 300 mg/kg APAP and (B) densitometry relative to control of Western blots shown in (A). (C) Western blot for p21 directly comparing 300 mg/kg and 600 mg/kg. (D) ELISA measuring CXCL14 plasma levels. (E) Immunohistochemistry for PCNA at 24 to 72h (200x) and corresponding PCNA+ hepatocyte counts per HPF (200x) (F). (G) Western blot of Klf6 and Cyclin D1 normalized to Gapdh. Data are presented as mean ± SEM. Statistical significance was assessed by two-way ANOVA with Sidak’s post hoc test. N=4-5 per group, *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: ELISA Assay The mouse CXCL14 ELISA kit was purchased from Novus Biologicals (#70015).

Techniques: Western Blot, Control, Enzyme-linked Immunosorbent Assay, Clinical Proteomics, Immunohistochemistry

Journal: Toxicology

Article Title: The p21 + Perinecrotic Hepatocytes Produce the Chemokine CXCL14 After a Severe Acetaminophen Overdose Promoting Hepatocyte Injury and Delaying Regeneration

doi: 10.1016/j.tox.2024.153804

Article Snippet: The mouse CXCL14 ELISA kit was purchased from Novus Biologicals (#70015).

Techniques: Immunohistochemistry, Western Blot, Expressing

Journal: Toxicology

Article Title: The p21 + Perinecrotic Hepatocytes Produce the Chemokine CXCL14 After a Severe Acetaminophen Overdose Promoting Hepatocyte Injury and Delaying Regeneration

doi: 10.1016/j.tox.2024.153804

Article Snippet: The mouse CXCL14 ELISA kit was purchased from Novus Biologicals (#70015).

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Toxicology

Article Title: The p21 + Perinecrotic Hepatocytes Produce the Chemokine CXCL14 After a Severe Acetaminophen Overdose Promoting Hepatocyte Injury and Delaying Regeneration

doi: 10.1016/j.tox.2024.153804

Article Snippet: The mouse CXCL14 ELISA kit was purchased from Novus Biologicals (#70015).

Techniques: Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry

Cxcl14 is expressed in muscle cells. ( a ) C2C12 cell media over the course of differentiation (0, 24, 48, 72 h) were subjected to ELISA assay to determine secreted Cxcl14 levels ( n =5, samples assayed in duplicates). ( b ) TA muscles were injured by BaCl 2 injection and isolated on days 3, 5, 7 and 14 after injury (AI). Upon cryosection, H&E staining or immunofluorescence staining for Cxcl14 (green) together with DAPI (blue) was performed ( n =3). Scale bars: 50 μm. ( c ) The procedure described in ( b ) was repeated. Muscle sections isolated on day 3 AI were immunofluorescently probed for MyoD (red), Cxcl14 (green) and DAPI (blue) ( n =6). Scale bar: 15 μm. Paired two-tailed t -test was performed for the data in ( a ). The data denoted by different letters ( a – c ) are significantly different from each other ( P <0.05). All error bars represent s.d. of independent replicates.

Journal: NPJ Regenerative Medicine

Article Title: Cxcl14 depletion accelerates skeletal myogenesis by promoting cell cycle withdrawal

doi: 10.1038/npjregenmed.2016.17

Figure Lengend Snippet: Cxcl14 is expressed in muscle cells. ( a ) C2C12 cell media over the course of differentiation (0, 24, 48, 72 h) were subjected to ELISA assay to determine secreted Cxcl14 levels ( n =5, samples assayed in duplicates). ( b ) TA muscles were injured by BaCl 2 injection and isolated on days 3, 5, 7 and 14 after injury (AI). Upon cryosection, H&E staining or immunofluorescence staining for Cxcl14 (green) together with DAPI (blue) was performed ( n =3). Scale bars: 50 μm. ( c ) The procedure described in ( b ) was repeated. Muscle sections isolated on day 3 AI were immunofluorescently probed for MyoD (red), Cxcl14 (green) and DAPI (blue) ( n =6). Scale bar: 15 μm. Paired two-tailed t -test was performed for the data in ( a ). The data denoted by different letters ( a – c ) are significantly different from each other ( P <0.05). All error bars represent s.d. of independent replicates.

Article Snippet: U0126 and ELISA kit for detection of mouse Cxcl14 was from Thermo Scientific.

Techniques: Enzyme-linked Immunosorbent Assay, Muscles, Injection, Isolation, Staining, Immunofluorescence, Two Tailed Test

Cxcl14 knockdown enhances C2C12 differentiation. ( a ) C2C12 cells were infected with lentiviruses expressing shCxcl14 or shScramble (negative control), selected for 2 days then differentiated for 72 h, followed by staining for MHC (green) and DAPI (pseudocoloured red) ( n =3). Scale bar: 50 μm. ( b ) Cells treated as in ( a ) were differentiated and at indicated time points of differentiation (‘Hrs diff’) were lysed and subjected to western analysis ( n =3). ( c ) Densitometry was performed on blots in ( b ), and the relative levels of proteins using tubulin as reference were quantified. Paired t test was performed to compare control and shCxcl14 at each time point. * P <0.05; ** P <0.01. ( d ) C2C12 cells were treated as in ( a ), then grown in the presence or absence of 25 ng/ml recombinant Cxcl14 (rCxcl14) for 24 h after selection. Cells were then differentiated for 3 days, followed by staining for MHC and DAPI and quantification of fusion index ( n =4). Scale bar: 50 μm. Paired two-tailed t -test was performed for the data in ( d ). The data denoted by different letters ( a – c ) are significantly different from each other ( P <0.05). All error bars represent s.d. of independent replicates.

Journal: NPJ Regenerative Medicine

Article Title: Cxcl14 depletion accelerates skeletal myogenesis by promoting cell cycle withdrawal

doi: 10.1038/npjregenmed.2016.17

Figure Lengend Snippet: Cxcl14 knockdown enhances C2C12 differentiation. ( a ) C2C12 cells were infected with lentiviruses expressing shCxcl14 or shScramble (negative control), selected for 2 days then differentiated for 72 h, followed by staining for MHC (green) and DAPI (pseudocoloured red) ( n =3). Scale bar: 50 μm. ( b ) Cells treated as in ( a ) were differentiated and at indicated time points of differentiation (‘Hrs diff’) were lysed and subjected to western analysis ( n =3). ( c ) Densitometry was performed on blots in ( b ), and the relative levels of proteins using tubulin as reference were quantified. Paired t test was performed to compare control and shCxcl14 at each time point. * P <0.05; ** P <0.01. ( d ) C2C12 cells were treated as in ( a ), then grown in the presence or absence of 25 ng/ml recombinant Cxcl14 (rCxcl14) for 24 h after selection. Cells were then differentiated for 3 days, followed by staining for MHC and DAPI and quantification of fusion index ( n =4). Scale bar: 50 μm. Paired two-tailed t -test was performed for the data in ( d ). The data denoted by different letters ( a – c ) are significantly different from each other ( P <0.05). All error bars represent s.d. of independent replicates.

Article Snippet: U0126 and ELISA kit for detection of mouse Cxcl14 was from Thermo Scientific.

Techniques: Knockdown, Infection, Expressing, Negative Control, Staining, Western Blot, Control, Recombinant, Selection, Two Tailed Test

Cxcl14 promotes cell cycle progression. ( a ) C2C12 cells were infected with lentiviruses expressing shCxcl14 or shScramble, selected for 2 days, grown in the presence or absence of 25 ng/ml rCxcl14 for 24 h, followed by differentiation for 24 h and subsequent staining with DAPI. Stained nuclei were counted ( n =4). ( b ) Cells were treated as in ( a ) but only stimulated with 10 ng/ml rCxcl14 for the indicated amount of time. Cells were then lysed and subjected to western analysis ( n =3). ( c ) Cells were treated as in ( a ) but differentiated for 24 h, followed by BrdU incorporation for 2 h. Cells were immunofluorescently stained for BrdU and DAPI, then counted ( n =5). ( d ) Cells were treated as in ( a ), followed by TUNEL assay to detect apoptotic cells ( n =3). ( e and f ) Cells were treated as in ( a ) but grown in the presence or absence of Ara-C for 24 h, then differentiated and immunofluorescently stained for MHC (green) or DAPI (pseudocoloured red). The fusion index was calculated ( n =3). Scale bar: 50 μm. Paired two-tailed t -test was performed for all the data. The data denoted by different letters ( a – c ) are significantly different from each other ( P <0.05). All error bars represent s.d. of independent replicates.

Journal: NPJ Regenerative Medicine

Article Title: Cxcl14 depletion accelerates skeletal myogenesis by promoting cell cycle withdrawal

doi: 10.1038/npjregenmed.2016.17

Figure Lengend Snippet: Cxcl14 promotes cell cycle progression. ( a ) C2C12 cells were infected with lentiviruses expressing shCxcl14 or shScramble, selected for 2 days, grown in the presence or absence of 25 ng/ml rCxcl14 for 24 h, followed by differentiation for 24 h and subsequent staining with DAPI. Stained nuclei were counted ( n =4). ( b ) Cells were treated as in ( a ) but only stimulated with 10 ng/ml rCxcl14 for the indicated amount of time. Cells were then lysed and subjected to western analysis ( n =3). ( c ) Cells were treated as in ( a ) but differentiated for 24 h, followed by BrdU incorporation for 2 h. Cells were immunofluorescently stained for BrdU and DAPI, then counted ( n =5). ( d ) Cells were treated as in ( a ), followed by TUNEL assay to detect apoptotic cells ( n =3). ( e and f ) Cells were treated as in ( a ) but grown in the presence or absence of Ara-C for 24 h, then differentiated and immunofluorescently stained for MHC (green) or DAPI (pseudocoloured red). The fusion index was calculated ( n =3). Scale bar: 50 μm. Paired two-tailed t -test was performed for all the data. The data denoted by different letters ( a – c ) are significantly different from each other ( P <0.05). All error bars represent s.d. of independent replicates.

Article Snippet: U0126 and ELISA kit for detection of mouse Cxcl14 was from Thermo Scientific.

Techniques: Infection, Expressing, Staining, Western Blot, BrdU Incorporation Assay, TUNEL Assay, Two Tailed Test

Cxcl14 knockdown accelerates muscle regeneration post-injury. ( a ) TA muscles were co-injected with BaCl 2 and shRNA viruses, and isolated on days 5, 7 and 14 after injury (AI). Upon cryosection, H&E staining was performed and regenerating myofiber cross-sectional area (CSA) was quantified ( n =6 for D5AI, n =8 for D7AI, n =7 for D14AI). ( b ) TA muscles injected as above were isolated on day 3 AI, cryosectioned and immunostained for Cxcl14 (green) along with DAPI (blue) ( n =3). Fluorescence intensity was quantified using ImageJ software. ( c – e ) TA muscle sections as described in ( b ) were immunostained for Ki-67 ( c ), MyoD ( d ) or myogenin ( e ), and the percentage of positive cells was quantified ( n =5). Scale bars: 50 μm. Paired two-tailed t -test was performed. The data denoted by different letters ( a – c ) are significantly different from each other ( P <0.05). All error bars represent s.d. of independent replicates.

Journal: NPJ Regenerative Medicine

Article Title: Cxcl14 depletion accelerates skeletal myogenesis by promoting cell cycle withdrawal

doi: 10.1038/npjregenmed.2016.17

Figure Lengend Snippet: Cxcl14 knockdown accelerates muscle regeneration post-injury. ( a ) TA muscles were co-injected with BaCl 2 and shRNA viruses, and isolated on days 5, 7 and 14 after injury (AI). Upon cryosection, H&E staining was performed and regenerating myofiber cross-sectional area (CSA) was quantified ( n =6 for D5AI, n =8 for D7AI, n =7 for D14AI). ( b ) TA muscles injected as above were isolated on day 3 AI, cryosectioned and immunostained for Cxcl14 (green) along with DAPI (blue) ( n =3). Fluorescence intensity was quantified using ImageJ software. ( c – e ) TA muscle sections as described in ( b ) were immunostained for Ki-67 ( c ), MyoD ( d ) or myogenin ( e ), and the percentage of positive cells was quantified ( n =5). Scale bars: 50 μm. Paired two-tailed t -test was performed. The data denoted by different letters ( a – c ) are significantly different from each other ( P <0.05). All error bars represent s.d. of independent replicates.

Article Snippet: U0126 and ELISA kit for detection of mouse Cxcl14 was from Thermo Scientific.

Techniques: Knockdown, Muscles, Injection, shRNA, Isolation, Staining, Fluorescence, Software, Two Tailed Test

Cxcl14 enhances muscle regeneration in aging mice. ( a ) TA muscles were injured with BaCl 2 , and isolated on day 7 AI. Upon cryosection, H&E staining was performed and regenerating myofiber cross-sectional area (CSA) was quantified ( n =12 for 4 months, n =9 for 6 months, n =11 for 8 monrhs, n =12 for 12 months, n =6 for 18 months). ( b ) TA muscle sections as described in ( a ) were isolated on day 7 AI, and the RNA was isolated and quantified by qRT-PCR. Horizontal bars represent the mean of individually plotted data points ( n =15 for 4 months, n =11 for 6 months, n =12 for 8 months, n =12 for 12 months, n =6 for 18 months). ( c ) TA muscles from 2-month-old (young) and 12-month-old (aged) mice were co-injected with Cxcl14 or scramble shRNA viruses together with BaCl 2 , then processed as in ( a ) at day 7 and day 14 AI ( n =6 for each). One-way ANOVA was used to analyze the data in ( b ) and paired two-tailed t -test was performed in ( c ). The data denoted by different letters ( a – c ) are significantly different from each other ( P <0.05). All error bars represent s.d. of independent replicates. Scale bar: 50 μm.

Journal: NPJ Regenerative Medicine

Article Title: Cxcl14 depletion accelerates skeletal myogenesis by promoting cell cycle withdrawal

doi: 10.1038/npjregenmed.2016.17

Figure Lengend Snippet: Cxcl14 enhances muscle regeneration in aging mice. ( a ) TA muscles were injured with BaCl 2 , and isolated on day 7 AI. Upon cryosection, H&E staining was performed and regenerating myofiber cross-sectional area (CSA) was quantified ( n =12 for 4 months, n =9 for 6 months, n =11 for 8 monrhs, n =12 for 12 months, n =6 for 18 months). ( b ) TA muscle sections as described in ( a ) were isolated on day 7 AI, and the RNA was isolated and quantified by qRT-PCR. Horizontal bars represent the mean of individually plotted data points ( n =15 for 4 months, n =11 for 6 months, n =12 for 8 months, n =12 for 12 months, n =6 for 18 months). ( c ) TA muscles from 2-month-old (young) and 12-month-old (aged) mice were co-injected with Cxcl14 or scramble shRNA viruses together with BaCl 2 , then processed as in ( a ) at day 7 and day 14 AI ( n =6 for each). One-way ANOVA was used to analyze the data in ( b ) and paired two-tailed t -test was performed in ( c ). The data denoted by different letters ( a – c ) are significantly different from each other ( P <0.05). All error bars represent s.d. of independent replicates. Scale bar: 50 μm.

Article Snippet: U0126 and ELISA kit for detection of mouse Cxcl14 was from Thermo Scientific.

Techniques: Muscles, Isolation, Staining, Quantitative RT-PCR, Injection, shRNA, Two Tailed Test

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